Update of the spectrum of GJB2 gene mutations in 152 Moroccan families with autosomal recessive nonsyndromic hearing loss.
Identifieur interne : 000464 ( Main/Exploration ); précédent : 000463; suivant : 000465Update of the spectrum of GJB2 gene mutations in 152 Moroccan families with autosomal recessive nonsyndromic hearing loss.
Auteurs : Amina Bakhchane [Maroc] ; Amale Bousfiha [Maroc] ; Hicham Charoute [Maroc] ; Sara Salime [Maroc] ; Mustapha Detsouli [Maroc] ; Khalid Snoussi [Maroc] ; Sellama Nadifi [Maroc] ; Mostafa Kabine [Maroc] ; Hassan Rouba [Maroc] ; Hind Dehbi [Maroc] ; Rachida Roky [Maroc] ; Majida Charif [Maroc] ; Abdelhamid Barakat [Maroc]Source :
- European journal of medical genetics [ 1878-0849 ] ; 2016.
Descripteurs français
- KwdFr :
- MESH :
- génétique : Connexines, Mutation, Surdité.
- physiopathologie : Surdité.
- épidémiologie : Surdité.
- Allèles, Femelle, Gènes récessifs, Humains, Hétérogénéité génétique, Maroc, Mâle.
- Wicri :
- geographic : Maroc.
English descriptors
- KwdEn :
- MESH :
Abstract
Deafness is one of the most common genetic diseases in humans and is subject to important genetic heterogeneity. The most common cause of non syndromic hearing loss (NSHL) is mutations in the GJB2 gene. This study aims to update and evaluate the spectrum of GJB2 allele variants in 152 Moroccan multiplex families with non syndromic hearing loss. Seven different mutations were detected: c.35delG, p.V37I, p.E47X, p.G200R, p.Del120E, p.R75Q, the last three mutations were described for the first time in Moroccan deaf patients, in addition to a novel nonsense mutation, the c.385G>T which is not referenced in any database. Sixty six families (43.42%) have mutations in the coding region of GJB2, while the homozygous c.35delG mutation still to date the most represented 51/152 (33.55%). The analysis of the geographical distribution of mutations located in GJB2 gene showed more allelic heterogeneity in the north and center compared to the south of Morocco. Our results showed that the GJB2 gene is a major contributor to non syndromic hearing loss in Morocco. Thus, this report of the GJB2 mutations spectrum all over Morocco has an important implication for establishing a suitable molecular diagnosis.
DOI: 10.1016/j.ejmg.2016.05.002
PubMed: 27169813
Affiliations:
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autosomal recessive nonsyndromic hearing loss.</title><author><name sortKey="Bakhchane, Amina" sort="Bakhchane, Amina" uniqKey="Bakhchane A" first="Amina" last="Bakhchane">Amina Bakhchane</name><affiliation wicri:level="1"><nlm:affiliation>Institut Pasteur, laboratoire de génétique moléculaire humaine, 20360 Casablanca, Morocco.</nlm:affiliation><country xml:lang="fr">Maroc</country><wicri:regionArea>Institut Pasteur, laboratoire de génétique moléculaire humaine, 20360 Casablanca</wicri:regionArea><wicri:noRegion>20360 Casablanca</wicri:noRegion></affiliation></author><author><name sortKey="Bousfiha, Amale" sort="Bousfiha, Amale" uniqKey="Bousfiha A" first="Amale" last="Bousfiha">Amale Bousfiha</name><affiliation wicri:level="1"><nlm:affiliation>Institut Pasteur, laboratoire de génétique moléculaire humaine, 20360 Casablanca, Morocco.</nlm:affiliation><country xml:lang="fr">Maroc</country><wicri:regionArea>Institut Pasteur, laboratoire de génétique moléculaire humaine, 20360 Casablanca</wicri:regionArea><wicri:noRegion>20360 Casablanca</wicri:noRegion></affiliation></author><author><name sortKey="Charoute, Hicham" sort="Charoute, Hicham" uniqKey="Charoute H" first="Hicham" last="Charoute">Hicham Charoute</name><affiliation wicri:level="1"><nlm:affiliation>Institut Pasteur, laboratoire de génétique moléculaire humaine, 20360 Casablanca, Morocco.</nlm:affiliation><country xml:lang="fr">Maroc</country><wicri:regionArea>Institut Pasteur, laboratoire de génétique moléculaire humaine, 20360 Casablanca</wicri:regionArea><wicri:noRegion>20360 Casablanca</wicri:noRegion></affiliation></author><author><name sortKey="Salime, Sara" sort="Salime, Sara" uniqKey="Salime S" first="Sara" last="Salime">Sara Salime</name><affiliation wicri:level="1"><nlm:affiliation>Institut Pasteur, laboratoire de génétique moléculaire humaine, 20360 Casablanca, Morocco.</nlm:affiliation><country xml:lang="fr">Maroc</country><wicri:regionArea>Institut Pasteur, laboratoire de génétique moléculaire humaine, 20360 Casablanca</wicri:regionArea><wicri:noRegion>20360 Casablanca</wicri:noRegion></affiliation></author><author><name sortKey="Detsouli, Mustapha" sort="Detsouli, Mustapha" uniqKey="Detsouli M" first="Mustapha" last="Detsouli">Mustapha Detsouli</name><affiliation wicri:level="1"><nlm:affiliation>Institut Pasteur, laboratoire de génétique moléculaire humaine, 20360 Casablanca, Morocco.</nlm:affiliation><country xml:lang="fr">Maroc</country><wicri:regionArea>Institut Pasteur, laboratoire de génétique moléculaire humaine, 20360 Casablanca</wicri:regionArea><wicri:noRegion>20360 Casablanca</wicri:noRegion></affiliation></author><author><name sortKey="Snoussi, Khalid" sort="Snoussi, Khalid" uniqKey="Snoussi K" first="Khalid" last="Snoussi">Khalid Snoussi</name><affiliation wicri:level="1"><nlm:affiliation>Institut Pasteur, laboratoire de génétique moléculaire humaine, 20360 Casablanca, Morocco.</nlm:affiliation><country xml:lang="fr">Maroc</country><wicri:regionArea>Institut Pasteur, laboratoire de génétique moléculaire humaine, 20360 Casablanca</wicri:regionArea><wicri:noRegion>20360 Casablanca</wicri:noRegion></affiliation></author><author><name sortKey="Nadifi, Sellama" sort="Nadifi, Sellama" uniqKey="Nadifi S" first="Sellama" last="Nadifi">Sellama Nadifi</name><affiliation wicri:level="1"><nlm:affiliation>Genetics and Molecular Pathology Laboratory, Medical School of Casablanca, Casablanca, Morocco.</nlm:affiliation><country xml:lang="fr">Maroc</country><wicri:regionArea>Genetics and Molecular Pathology Laboratory, Medical School of Casablanca, Casablanca</wicri:regionArea><wicri:noRegion>Casablanca</wicri:noRegion></affiliation></author><author><name sortKey="Kabine, Mostafa" sort="Kabine, Mostafa" uniqKey="Kabine M" first="Mostafa" last="Kabine">Mostafa Kabine</name><affiliation wicri:level="1"><nlm:affiliation>Laboratoire de Biochimie et Biologie Moléculaire, Université Hassan II, Faculté des Sciences Aïn Chock, Casablanca, Morocco.</nlm:affiliation><country xml:lang="fr">Maroc</country><wicri:regionArea>Laboratoire de Biochimie et Biologie Moléculaire, Université Hassan II, Faculté des Sciences Aïn Chock, Casablanca</wicri:regionArea><wicri:noRegion>Casablanca</wicri:noRegion></affiliation></author><author><name sortKey="Rouba, Hassan" sort="Rouba, Hassan" uniqKey="Rouba H" first="Hassan" last="Rouba">Hassan Rouba</name><affiliation wicri:level="1"><nlm:affiliation>Institut Pasteur, laboratoire de génétique moléculaire humaine, 20360 Casablanca, Morocco.</nlm:affiliation><country xml:lang="fr">Maroc</country><wicri:regionArea>Institut Pasteur, laboratoire de génétique moléculaire humaine, 20360 Casablanca</wicri:regionArea><wicri:noRegion>20360 Casablanca</wicri:noRegion></affiliation></author><author><name sortKey="Dehbi, Hind" sort="Dehbi, Hind" uniqKey="Dehbi H" first="Hind" last="Dehbi">Hind Dehbi</name><affiliation wicri:level="1"><nlm:affiliation>Genetics and Molecular Pathology Laboratory, Medical School of Casablanca, Casablanca, Morocco.</nlm:affiliation><country xml:lang="fr">Maroc</country><wicri:regionArea>Genetics and Molecular Pathology Laboratory, Medical School of Casablanca, Casablanca</wicri:regionArea><wicri:noRegion>Casablanca</wicri:noRegion></affiliation></author><author><name sortKey="Roky, Rachida" sort="Roky, Rachida" uniqKey="Roky R" first="Rachida" last="Roky">Rachida Roky</name><affiliation wicri:level="1"><nlm:affiliation>Université Hassan II Ain Chock, Laboratoire de Physiologie et génétique moléculaire, Km 8 Route d'El Jadida, B.P 5366 Maarif, Casablanca, 20100, Morocco.</nlm:affiliation><country xml:lang="fr">Maroc</country><wicri:regionArea>Université Hassan II Ain Chock, Laboratoire de Physiologie et génétique moléculaire, Km 8 Route d'El Jadida, B.P 5366 Maarif, Casablanca, 20100</wicri:regionArea><wicri:noRegion>20100</wicri:noRegion></affiliation></author><author><name sortKey="Charif, Majida" sort="Charif, Majida" uniqKey="Charif M" first="Majida" last="Charif">Majida Charif</name><affiliation wicri:level="1"><nlm:affiliation>Institut Pasteur, laboratoire de génétique moléculaire humaine, 20360 Casablanca, Morocco.</nlm:affiliation><country xml:lang="fr">Maroc</country><wicri:regionArea>Institut Pasteur, laboratoire de génétique moléculaire humaine, 20360 Casablanca</wicri:regionArea><wicri:noRegion>20360 Casablanca</wicri:noRegion></affiliation></author><author><name sortKey="Barakat, Abdelhamid" sort="Barakat, Abdelhamid" uniqKey="Barakat A" first="Abdelhamid" last="Barakat">Abdelhamid Barakat</name><affiliation wicri:level="1"><nlm:affiliation>Institut Pasteur, laboratoire de génétique moléculaire humaine, 20360 Casablanca, Morocco. Electronic address: hamid.barakat@pasteur.ma.</nlm:affiliation><country xml:lang="fr">Maroc</country><wicri:regionArea>Institut Pasteur, laboratoire de génétique moléculaire humaine, 20360 Casablanca</wicri:regionArea><wicri:noRegion>20360 Casablanca</wicri:noRegion></affiliation></author></analytic><series><title level="j">European journal of medical genetics</title><idno type="eISSN">1878-0849</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Alleles (MeSH)</term><term>Connexins (genetics)</term><term>Deafness (epidemiology)</term><term>Deafness (genetics)</term><term>Deafness (physiopathology)</term><term>Female (MeSH)</term><term>Genes, Recessive (MeSH)</term><term>Genetic Heterogeneity (MeSH)</term><term>Humans (MeSH)</term><term>Male (MeSH)</term><term>Morocco (MeSH)</term><term>Mutation (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Allèles (MeSH)</term><term>Connexines (génétique)</term><term>Femelle (MeSH)</term><term>Gènes récessifs (MeSH)</term><term>Humains (MeSH)</term><term>Hétérogénéité génétique (MeSH)</term><term>Maroc (MeSH)</term><term>Mutation (génétique)</term><term>Mâle (MeSH)</term><term>Surdité (génétique)</term><term>Surdité (physiopathologie)</term><term>Surdité (épidémiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Connexins</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Morocco</term></keywords><keywords scheme="MESH" qualifier="epidemiology" xml:lang="en"><term>Deafness</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Deafness</term><term>Mutation</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Connexines</term><term>Mutation</term><term>Surdité</term></keywords><keywords scheme="MESH" qualifier="physiopathologie" xml:lang="fr"><term>Surdité</term></keywords><keywords scheme="MESH" qualifier="physiopathology" xml:lang="en"><term>Deafness</term></keywords><keywords scheme="MESH" qualifier="épidémiologie" xml:lang="fr"><term>Surdité</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Alleles</term><term>Female</term><term>Genes, Recessive</term><term>Genetic Heterogeneity</term><term>Humans</term><term>Male</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Allèles</term><term>Femelle</term><term>Gènes récessifs</term><term>Humains</term><term>Hétérogénéité génétique</term><term>Maroc</term><term>Mâle</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Maroc</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Deafness is one of the most common genetic diseases in humans and is subject to important genetic heterogeneity. The most common cause of non syndromic hearing loss (NSHL) is mutations in the GJB2 gene. This study aims to update and evaluate the spectrum of GJB2 allele variants in 152 Moroccan multiplex families with non syndromic hearing loss. Seven different mutations were detected: c.35delG, p.V37I, p.E47X, p.G200R, p.Del120E, p.R75Q, the last three mutations were described for the first time in Moroccan deaf patients, in addition to a novel nonsense mutation, the c.385G>T which is not referenced in any database. Sixty six families (43.42%) have mutations in the coding region of GJB2, while the homozygous c.35delG mutation still to date the most represented 51/152 (33.55%). The analysis of the geographical distribution of mutations located in GJB2 gene showed more allelic heterogeneity in the north and center compared to the south of Morocco. Our results showed that the GJB2 gene is a major contributor to non syndromic hearing loss in Morocco. Thus, this report of the GJB2 mutations spectrum all over Morocco has an important implication for establishing a suitable molecular diagnosis. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">27169813</PMID><DateCompleted><Year>2017</Year><Month>02</Month><Day>10</Day></DateCompleted><DateRevised><Year>2020</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1878-0849</ISSN><JournalIssue CitedMedium="Internet"><Volume>59</Volume><Issue>6-7</Issue><PubDate><Year>2016</Year><Month>Jun</Month></PubDate></JournalIssue><Title>European journal of medical genetics</Title><ISOAbbreviation>Eur J Med Genet</ISOAbbreviation></Journal><ArticleTitle>Update of the spectrum of GJB2 gene mutations in 152 Moroccan families with autosomal recessive nonsyndromic hearing loss.</ArticleTitle><Pagination><MedlinePgn>325-9</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.ejmg.2016.05.002</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S1769-7212(16)30064-7</ELocationID><Abstract><AbstractText>Deafness is one of the most common genetic diseases in humans and is subject to important genetic heterogeneity. The most common cause of non syndromic hearing loss (NSHL) is mutations in the GJB2 gene. This study aims to update and evaluate the spectrum of GJB2 allele variants in 152 Moroccan multiplex families with non syndromic hearing loss. Seven different mutations were detected: c.35delG, p.V37I, p.E47X, p.G200R, p.Del120E, p.R75Q, the last three mutations were described for the first time in Moroccan deaf patients, in addition to a novel nonsense mutation, the c.385G>T which is not referenced in any database. Sixty six families (43.42%) have mutations in the coding region of GJB2, while the homozygous c.35delG mutation still to date the most represented 51/152 (33.55%). The analysis of the geographical distribution of mutations located in GJB2 gene showed more allelic heterogeneity in the north and center compared to the south of Morocco. Our results showed that the GJB2 gene is a major contributor to non syndromic hearing loss in Morocco. Thus, this report of the GJB2 mutations spectrum all over Morocco has an important implication for establishing a suitable molecular diagnosis. </AbstractText><CopyrightInformation>Copyright © 2016 Elsevier Masson SAS. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Bakhchane</LastName><ForeName>Amina</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Institut Pasteur, laboratoire de génétique moléculaire humaine, 20360 Casablanca, Morocco.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bousfiha</LastName><ForeName>Amale</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Institut Pasteur, laboratoire de génétique moléculaire humaine, 20360 Casablanca, Morocco.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Charoute</LastName><ForeName>Hicham</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Institut Pasteur, laboratoire de génétique moléculaire humaine, 20360 Casablanca, Morocco.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Salime</LastName><ForeName>Sara</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Institut Pasteur, laboratoire de génétique moléculaire humaine, 20360 Casablanca, Morocco.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Detsouli</LastName><ForeName>Mustapha</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Institut Pasteur, laboratoire de génétique moléculaire humaine, 20360 Casablanca, Morocco.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Snoussi</LastName><ForeName>Khalid</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Institut Pasteur, laboratoire de génétique moléculaire humaine, 20360 Casablanca, Morocco.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Nadifi</LastName><ForeName>Sellama</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Genetics and Molecular Pathology Laboratory, Medical School of Casablanca, Casablanca, Morocco.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kabine</LastName><ForeName>Mostafa</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Laboratoire de Biochimie et Biologie Moléculaire, Université Hassan II, Faculté des Sciences Aïn Chock, Casablanca, Morocco.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rouba</LastName><ForeName>Hassan</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Institut Pasteur, laboratoire de génétique moléculaire humaine, 20360 Casablanca, Morocco.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dehbi</LastName><ForeName>Hind</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Genetics and Molecular Pathology Laboratory, Medical School of Casablanca, Casablanca, Morocco.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Roky</LastName><ForeName>Rachida</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Université Hassan II Ain Chock, Laboratoire de Physiologie et génétique moléculaire, Km 8 Route d'El Jadida, B.P 5366 Maarif, Casablanca, 20100, Morocco.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Charif</LastName><ForeName>Majida</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Institut Pasteur, laboratoire de génétique moléculaire humaine, 20360 Casablanca, Morocco.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Barakat</LastName><ForeName>Abdelhamid</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Institut Pasteur, laboratoire de génétique moléculaire humaine, 20360 Casablanca, Morocco. Electronic address: hamid.barakat@pasteur.ma.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>05</Month><Day>08</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Eur J Med Genet</MedlineTA><NlmUniqueID>101247089</NlmUniqueID><ISSNLinking>1769-7212</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D017630">Connexins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C000607042">GJB2 protein, human</NameOfSubstance></Chemical></ChemicalList><SupplMeshList><SupplMeshName Type="Disease" UI="C580334">Nonsyndromic Deafness</SupplMeshName></SupplMeshList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000483" MajorTopicYN="N">Alleles</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017630" MajorTopicYN="N">Connexins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003638" MajorTopicYN="N">Deafness</DescriptorName><QualifierName UI="Q000453" MajorTopicYN="N">epidemiology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000503" MajorTopicYN="N">physiopathology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005808" MajorTopicYN="N">Genes, Recessive</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018740" MajorTopicYN="Y">Genetic Heterogeneity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009018" MajorTopicYN="N" Type="Geographic">Morocco</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009154" MajorTopicYN="N">Mutation</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">GJB2</Keyword><Keyword MajorTopicYN="N">Hearing loss</Keyword><Keyword MajorTopicYN="N">Morocco</Keyword><Keyword MajorTopicYN="N">Mutation</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2015</Year><Month>12</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2016</Year><Month>04</Month><Day>26</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2016</Year><Month>05</Month><Day>04</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>5</Month><Day>13</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>5</Month><Day>14</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2017</Year><Month>2</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">27169813</ArticleId><ArticleId IdType="pii">S1769-7212(16)30064-7</ArticleId><ArticleId IdType="doi">10.1016/j.ejmg.2016.05.002</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Maroc</li></country></list><tree><country name="Maroc"><noRegion><name sortKey="Bakhchane, Amina" sort="Bakhchane, Amina" uniqKey="Bakhchane A" first="Amina" last="Bakhchane">Amina Bakhchane</name></noRegion><name sortKey="Barakat, Abdelhamid" sort="Barakat, Abdelhamid" uniqKey="Barakat A" first="Abdelhamid" last="Barakat">Abdelhamid Barakat</name><name sortKey="Bousfiha, Amale" sort="Bousfiha, Amale" uniqKey="Bousfiha A" first="Amale" last="Bousfiha">Amale Bousfiha</name><name sortKey="Charif, Majida" sort="Charif, Majida" uniqKey="Charif M" first="Majida" last="Charif">Majida Charif</name><name sortKey="Charoute, Hicham" sort="Charoute, Hicham" uniqKey="Charoute H" first="Hicham" last="Charoute">Hicham Charoute</name><name sortKey="Dehbi, Hind" sort="Dehbi, Hind" uniqKey="Dehbi H" first="Hind" last="Dehbi">Hind Dehbi</name><name sortKey="Detsouli, Mustapha" sort="Detsouli, Mustapha" uniqKey="Detsouli M" first="Mustapha" last="Detsouli">Mustapha Detsouli</name><name sortKey="Kabine, Mostafa" sort="Kabine, Mostafa" uniqKey="Kabine M" first="Mostafa" last="Kabine">Mostafa Kabine</name><name sortKey="Nadifi, Sellama" sort="Nadifi, Sellama" uniqKey="Nadifi S" first="Sellama" last="Nadifi">Sellama Nadifi</name><name sortKey="Roky, Rachida" sort="Roky, Rachida" uniqKey="Roky R" first="Rachida" last="Roky">Rachida Roky</name><name sortKey="Rouba, Hassan" sort="Rouba, Hassan" uniqKey="Rouba H" first="Hassan" last="Rouba">Hassan Rouba</name><name sortKey="Salime, Sara" sort="Salime, Sara" uniqKey="Salime S" first="Sara" last="Salime">Sara Salime</name><name sortKey="Snoussi, Khalid" sort="Snoussi, Khalid" uniqKey="Snoussi K" first="Khalid" last="Snoussi">Khalid Snoussi</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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